Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Topical Application of the Antimicrobial Agent Triclosan Induces NLRP3 Inflammasome Activation and Mitochondrial Dysfunction

doi: 10.1093/toxsci/kfaa056

Figure Lengend Snippet: Primary and Secondary Antibodies Used for ProteinSimple Wes Immunoassay

Article Snippet: Total protein was used as a loading control and for normalization. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Reference Sample Concentration Antibody Dilution Secondary Antibody Opa1 Novus Biologicals 0.2 mg/ml (skin) 1:200 (skin) Anti-rabbit-HRP (Wes detection kit) NB-110-55290SS 0.2 mg/ml (dLN) 1:200 (dLN) Drp1 Novus Biologicals 0.1 mg/ml (skin) 1:150 (skin) NB110-55288SS 0.1 mg/ml (dLN) 1:150 (dLN) Open in a separate window Primary and Secondary Antibodies Used for ProteinSimple Wes Immunoassay Statistical analysis A one-way analysis of variance (ANOVA) was conducted followed by a Dunnett’s multiple comparison post-test to compare groups to acetone control.

Techniques: Concentration Assay

Journal: Oncotarget

Article Title: To unite or divide: mitochondrial dynamics in the murine outer retina that preceded age related photoreceptor loss

doi:

Figure Lengend Snippet: A. Representative micrographs of retinal sections immunostained with a fusion marker, Opa1 (red) and the nuclei counterstained with 4′, 6-diamino-2-phenylindole (DAPI) (Blue). Opa1 is present in the ganglion cell layer and in the outer plexiform layer of young mice while in the old it is present in the outer plexiform layer and the photoreceptor layer. B. Opa1 is expressed less in the old than the young retina. C. Opa1 expression is significantly higher in the outer plexiform layer of the old retina compared to the young ( P = 0.0048). D. Opa1 expression in the photoreceptor layer. Opa1 is strongly expressed in the photoreceptor layer of the old retina when compared to the young ( P = 0.0048). E. Western blot measurements of Opa1 protein revealing that it is reduced in the old retina compared to young but this did not reach statistical significance ( P = 0.1276). Mean ± SEM. Scale bar = 25μm. GCL; ganglion cell layer, IPL; Inner plexiform layer, INL; inner nuclear layer, OPL; outer plexiform layer, ONL; outer nuclear layer, PR; photoreceptor layer and RPE; retinal pigment epithelial.

Article Snippet: They were then incubated overnight with either a rabbit polyclonal antibody to Fis1 (1:200, Abcam), a rabbit polyclonal antibody to Opa1 (1:300, Abcam), a rabbit polyclonal antibody to heat shock protein 60 (Hsp60) or a goat polyclonal antibody to cytochrome c oxidase subunit 3 (COX III) (1:100, Santa Cruz Biotechnology, Inc.) diluted in 1% Normal Donkey Serum in 0.3% Triton X-100 in PBS.

Techniques: Marker, Expressing, Western Blot

Journal: Cell reports

Article Title: Opa1 and Drp1 reciprocally regulate cristae morphology, ETC function, and NAD + regeneration in KRas-mutant lung adenocarcinoma

doi: 10.1016/j.celrep.2022.111818

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Opa1 (IHC) , GeneTex , Cat# 48589; RRID: AB_10623460.

Techniques: Western Blot, Virus, Plasmid Preparation, Recombinant, Luciferase, Software

Journal: Applied Bionics and Biomechanics

Article Title: Serum Levels of Mitochondrial Fission- and Fusion-Related Genes of Coal Workers' Pneumoconiosis and Risk Factor Analysis Based on a Generalized Linear Model

doi: 10.1155/2022/8629583

Figure Lengend Snippet: Relative protein expression levels of fission- and fusion-related genes in serum of coal mine workers. (a) Expression of mitochondrial fission- and fusion-related genes and proteins in serum by western blot analysis. (b) The relative protein expression level of OPA1. (c) The relative protein expression level of Drp1. (d) The relative protein expression levels of MFN2. (e) The relative protein expression level of Fis1. a P < 0.05, compared with the healthy control group. b P < 0.05, compared with the dust exposure control group.

Article Snippet: Rabbit anti-MFN2 polyclonal antibody (sc-515647, Santa Cruz Biotechnology, Inc. USA), rabbit anti-Drp1 polyclonal antibody (8570S, Santa Cruz Biotechnology, Inc. USA), rabbit anti-OPA1 polyclonal antibody (67589S, Santa Cruz Biotechnology, Inc. USA), rabbit anti-Fis1 polyclonal antibody (sc-376446, Santa Cruz Biotechnology, Inc., USA), Rabbit anti-Col I (collagen type I) polyclonal antibody (AF7001, Affinity Biosciences, Inc., Jiangsu, China), rabbit anti-Col III (collagen type III) polyclonal antibody (AF0136, Affinity Biosciences, Inc., Jiangsu, China), rabbit anti- α -SMA ( α -smooth muscle actin) polyclonal antibody (ab5694, Abcam Biotechnology, Inc., USA), and goat anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP (ZB-2301, ZB-2305, Beijing Zhongshanjinqiao Biotechnology Co., Ltd., Beijing, China) were utilized.

Techniques: Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Adaptive effect of sericin on hepatic mitochondrial conformation through its regulation of apoptosis, autophagy and energy maintenance: a proteomics approach

doi: 10.1038/s41598-018-33372-4

Figure Lengend Snippet: OPA1 immunogold labelling at each stage of mitochondrial degeneration. OPA1 ( a–k ) gold labelling at all stages of the liver mitochondria: intact ( a,f ), swelling ( b,g ), spheroid ( c,h ), clumping ( d , i ) and ghost ( e , j ). Data in the bar graph is represented by mean ± SEM; **p < 0.01 and ***p < 0.001.

Article Snippet: In this study, several markers (MyBioSource, USA) were used as primary antibodies: apoptotic markers, mouse polyclonal NDUFS1 and caspase-3; autophagic markers, mouse polyclonal TUFM and rabbit polyclonal anti-LC3; fusion and fission markers, rabbit polyclonal OPA1 and DRP1 and energy marker, rabbit polyclonal anti-HDHD3.

Techniques: